Creatinine Clearance Experiment

This lab included various experiments that demonstrated how renal system responses and reacts when subjects consuming different types of fluids. Respective fluid volumes are based on each subject’s body mass. Based on each subject’s body mass, after converted from pounds to kilograms, every subjects has different volume and fluids for ingestion. For the control subject, she ingested no fluid throughout entire lab which represented the conditions of normal fluid. For the hypotonic subjects, she ingested 854mL of water within 10 minutes which represented the dilution of bodily fluids by seeing the urine became clear and clear each time. For isotonic subjects, who consumed 1120mL of isotonic saline (0.9% NaCl), shown the importance of sodium related to normal body composition. For alkalosis subject, who consumed 100mL of sodium …show more content…
So, creatinine clearance needs to be calculated in order to estimate GFR. Since clearance defined as the volume of plasma that is completely cleared or cleaned by the kidney when excreting substance in urine, it is a good way to determine if the kidneys are functioning properly. Creatinine is a metabolic byproduct of skeletal muscle that is freely filtered, not re-absorbed and with little secretion. Although creatinine clearance is not completely accurate when measuring GFR, it provides better and closer approximation than insulin clearance. GFR is the volume of filtrate formed per unit time. By comparing the clearance of creatinine to GFR, it was able to determine whether there was any reabsorption or secretion of creatinine by the kidney. If a substance is filtered at the glomerulus and fully, completely reabsorbed and not secreted, the clearance will be zero. If a substance is filtered and unmodified by the nephron, clearance will equal GFR. Clearance will be greater than GFR when a substance is filtered and secreted, but not reabsorbed (Sherwood,