SDS-Page/Coomassie Blue Analysis: Make a stacking gel for the SDS Page. Then, make the 12% resolving gel by using deionized water with 4X resolving buffer, 10% APS, 30% Acrylamide, and TEMED in a vial. Pour the stacking gel on top of the resolving gel and then insert the plastic comb to show the wells. Place the gel in an electrophoresis tank that is filled with 500 mL of electrophoresis buffer. Vortex, boil, and centrifuge the G0, G3, GCE, W4, W4, E3, E2 samples, and the ladder. Load the samples into the wells and then run the gel at 200 volts for 45 minutes (or) until the bromophenol blue reaches the bottom of the page. After the gel is removed, stain it with Coomassie blue solution.
