Aromatic plants bear essential oils which are fragrant and so are named ‘Aromatic’. These plants retain their fragrant components in specialised cells and tissues of organs even after drying. Oil may be present in external glands as epidermal cells or their modification such as ‘Secretory Hair’ or may be present internally throughout the plant forming Schizogenous formation which on dissolution of surrounding cells forms Schizolysogenous gland which further grow into long canals. Secretion such as oils and resins forms in epithelial cells passes through the cell wall into interior of the gland where it is stored. These plants are the source of secondary metabolites such as flavanoids, polyphenols, tannins, etc. and are therefore used as a
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• To carry out chemical profiling and antioxidant activity of essential oils extracted from two sample trees of Cinnamomum tamala.
• To observe antimicrobial activity of essential oils and extracts of Cinnamon on various food borne human pathogenic bacteria.
Materials and Method:
4.1 Plant material
Aerial parts (needles, branchlets and female cones) of C. tamala were collected from Centre for Aromatic Plants premises (680 m asl) in March 2015. The plant specimen was identified based on the herbarium records with Centre for Aromatic Plants (CAP), Selaqui, Dehradun (India).
Fig no: Fresh and dried leaf collected from two different trees of Cinnamomum tamala
4.2 Preparation of extracts:
• Samples taken from the trees were shade dried for ten days before carrying out the experiments.
• After drying of leaves, they were pulverized.
• From the pulverized samples, 10 gm of material was weighed and soaked in 100 ml of each Acetone, methanol and water for at least 24 hours(aqueous) and to a maximum of 72 hours(methanolic and acetone). Fig : Extracts and Essential Oil of Cinnamomum tamala …show more content…
The column was Rtx-5 capillary columns (60 m ´ 0.32 mm, 0.25 μm film thickness). Helium (He) was the carrier gas at a flow rate 1.0 mL/min. The GC was interfaced with (Perkin Elmer Clarus 500) mass detector operating in the EI+ mode. The mass spectra were recorded over 40-500 amu that revealed the total ion current (TIC) chromatograms, using an ionizing voltage of 70 eV. Temperature program was used as the same as described above for GC analyses. The temperatures of the injector, transfer line and ion source were maintained at 210°C, 210°C and 200°C, respectively.
4.6 Identification of compounds
Identification of the components was done on the basis of retention time, retention indices, determined with reference to homologous series of n-alkanes (C8-C24 Sigma-Aldrich) under identical experimental conditions, co-injection with authentic standard compounds, mass spectra with the library provided by instrument software (NIST/ Wiley) and by comparing their mass spectra with those reported in literature (Adams, 2009). Quantification of each compound was performed on the basis of their GC peak area, using the normalization procedure without using correction factors.
Anti microbial activity:
• To carry out chemical profiling and antioxidant activity of essential oils extracted from two sample trees of Cinnamomum tamala.
• To observe antimicrobial activity of essential oils and extracts of Cinnamon on various food borne human pathogenic bacteria.
Materials and Method:
4.1 Plant material
Aerial parts (needles, branchlets and female cones) of C. tamala were collected from Centre for Aromatic Plants premises (680 m asl) in March 2015. The plant specimen was identified based on the herbarium records with Centre for Aromatic Plants (CAP), Selaqui, Dehradun (India).
Fig no: Fresh and dried leaf collected from two different trees of Cinnamomum tamala
4.2 Preparation of extracts:
• Samples taken from the trees were shade dried for ten days before carrying out the experiments.
• After drying of leaves, they were pulverized.
• From the pulverized samples, 10 gm of material was weighed and soaked in 100 ml of each Acetone, methanol and water for at least 24 hours(aqueous) and to a maximum of 72 hours(methanolic and acetone). Fig : Extracts and Essential Oil of Cinnamomum tamala …show more content…
The column was Rtx-5 capillary columns (60 m ´ 0.32 mm, 0.25 μm film thickness). Helium (He) was the carrier gas at a flow rate 1.0 mL/min. The GC was interfaced with (Perkin Elmer Clarus 500) mass detector operating in the EI+ mode. The mass spectra were recorded over 40-500 amu that revealed the total ion current (TIC) chromatograms, using an ionizing voltage of 70 eV. Temperature program was used as the same as described above for GC analyses. The temperatures of the injector, transfer line and ion source were maintained at 210°C, 210°C and 200°C, respectively.
4.6 Identification of compounds
Identification of the components was done on the basis of retention time, retention indices, determined with reference to homologous series of n-alkanes (C8-C24 Sigma-Aldrich) under identical experimental conditions, co-injection with authentic standard compounds, mass spectra with the library provided by instrument software (NIST/ Wiley) and by comparing their mass spectra with those reported in literature (Adams, 2009). Quantification of each compound was performed on the basis of their GC peak area, using the normalization procedure without using correction factors.
Anti microbial activity: