The purpose of this experiment is to determine the optimal absorption of different solutions, specifically the Chloroplast extract (chlorophyll a and chlorophyll b) and Fast Green solution using the technique of spectroscopy. The following graphs and data will be used to identify the concentration of the given unknown solution #294. Spectroscopy is a process whereby a spectrophotometer is used to determine the absorbance levels of different solutions at different wavelengths (Pettruci et al., 2011). Essentially, it studies the area of science involving the visible light spectrum, which the a portion of the electromagnetic spectrum the the human eye can see (Mantele, 2016). This spectrum is made up of range of wavelengths (400 nm - 750 nm),
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Chloropyll is a light absorbing pigment found in chloroplasts, that is crucial to photosynthesis. (Petrucci et al., 2011). Chloroplasts, which are organelles found in plants, are thus, responsible for photosynthesis (Alberts et al., 2010). The technique of chromatography, which is the process of separating molecules in a mixture, is used for this experiment (Alberts et al., 2010). This is where chloroplast extract is applied to the filter paper, producing a chromatogram containing different colours signifying different types of pigments (Alberts et al., 2010). The colour of the pigment present on the chromatogram signifies that it was unable to absorb that specific colour (Alberts et al., 2010). For example, chlorophyll has a green pigment, because it is unable to absorb the colour green (Petrucci et al., 2011). This lab uses a chloroplast extract that is separated into four different pigments, including chlorophyll a, chlorophyll b, carotenes and xanthophylls (Alberts et al., 2010). From the graph, both the chlorophyll a and b are cut into small squares and immersed into a test tube. The coloured liquid was then retrieved and inserted into he spectrophotometer to produce an absorption spectrum, and thus retrieve the maximum absorption of both chlorophyll’s a
Chloropyll is a light absorbing pigment found in chloroplasts, that is crucial to photosynthesis. (Petrucci et al., 2011). Chloroplasts, which are organelles found in plants, are thus, responsible for photosynthesis (Alberts et al., 2010). The technique of chromatography, which is the process of separating molecules in a mixture, is used for this experiment (Alberts et al., 2010). This is where chloroplast extract is applied to the filter paper, producing a chromatogram containing different colours signifying different types of pigments (Alberts et al., 2010). The colour of the pigment present on the chromatogram signifies that it was unable to absorb that specific colour (Alberts et al., 2010). For example, chlorophyll has a green pigment, because it is unable to absorb the colour green (Petrucci et al., 2011). This lab uses a chloroplast extract that is separated into four different pigments, including chlorophyll a, chlorophyll b, carotenes and xanthophylls (Alberts et al., 2010). From the graph, both the chlorophyll a and b are cut into small squares and immersed into a test tube. The coloured liquid was then retrieved and inserted into he spectrophotometer to produce an absorption spectrum, and thus retrieve the maximum absorption of both chlorophyll’s a