The objective of the experiment was to separate chlorophyll a and chlorophyll b and the alpha and beta conformations using the paper and column chromatography. The two techniques that were used to separate and identify the plant pigments were the paper chromatography and column chromatography. For the mixture to be separated, it must first be applied to a stationary phase. The stationary phase in the paper chromatography experiment was the filter paper. The mobile phase used in this experiment to help separate the pigments were the hexane and acetone solution. Boiling the spinach leaves helps to release the pigments by breaking down the cellulose. After adding the spinach leaves and the solution, there was not a lot of extraction collected
