Background
All cells are surrounded by a barrier called the cell membrane. It regulates what enters and leaves the cell, and also protects and supports the cell. Cell membranes are semipermeable, meaning it doesn’t allow all molecules to pass through it. For example, polar molecules and differently charged molecules are blocked from passing through the cell membrane, while smaller molecules can easily pass through it. Large polar molecules and ions can pass through the membrane only by passive or …show more content…
2. Fill a plastic cup with distilled water. Put a piece of dialysis tubing in the water. Leave the tubing in for at least 30 seconds.
3. Label your three pipettes: 20% sucrose, 40% sucrose and distilled water. Next, label a cup with the name of Solution A for Experiment 1 (see chart above).
4. Add 150 ml of Solution A to the labeled cup.
5. Remove the dialysis tubing from the water, and gently roll one end of the tubing until you get it open. Continue rolling down the tubing until it is open completely.
6. Use string or tie a knot in one end of the dialysis tubing. This should be as close to the end as possible.
7. Using a graduated pipet, place 10 ml of Solution B inside the dialysis tubing. Carefully squeeze the remaining, empty length of the tubing to remove as much air as possible.
8. Tie a knot in the open end of the tubing, as close to the end as possible.
9. Rinse the dialysis tubing with distilled water, then gently pat the tubing dry with a paper towel.
10. Measure the mass of the tubing with Solution B and record your …show more content…
Gently swirl the solutions and then record the color of each solution.
Iodine tests for starch. A blue-black color indicates that starch is present, while a brown-amber color indicates that it’s not.
Fill a plastic cup with distilled water, and place a piece of dialysis tubing in the water for at least 30 seconds.
Add 150 mL of distilled water to a second cup.
Remove the dialysis tubing from the water, and gently roll an end of the tubing between your fingers to open the tubing. Continue down the length of the tubing to open it completely. Tie a knot in one end of the tubing, as close to the end as possible.
Place 10 mL of the starch-glucose mixture inside the dialysis tubing using a pipet. Carefully squeeze the remaining, empty length of the tubing. Then, tie a knot in the open end of the tubing.
Rinse the dialysis tubing with distilled water, and gently pat it dry with a paper towel.
Place the filled tubing into the cup with 150 mL of distilled water. Let it sit for 15 minutes.
After 15 minutes, removing the tubing from the water, and rinse the tubing with the distilled water. Pat it dry with a paper towel.
Number four test tubes 1 through