Other researchers had already determined that paxillin contributes greatly to the structural integrity of the outer membrane of a cell. For instance, paxillin leads to the formation of actin structures that are located at the edge of a cell, such as lamellipodia. What scientist don’t know is exactly how paxillin effects different functions in a cell. In order to solve this mystery, Cress, et al. wanted to take the research further and determine the role that paxillin played in lamellipodia formation, and how paxillin affected the barrier that surrounds a cell. This would be a step toward finding out how much of an effect that proteins have in the restoration of the outer barrier of a cell. If scientist could figure this out, another step could be taken to figure out how to use proteins to fix cells. They did not want to test this theory on all types of cells, so they chose to only focus on human lung microvascular endothelial cells, or HLMVEC for short. That means that this study could really only directly help with the study of damaged lung cells. They also wanted to focus on how paxillin was effected by Y31 and Y118, which are specific amino acids that are used by cells to synthesize proteins. The scientists needed to know, if their theory was correct and paxillin had an effect on the barrier of a cell, how to synthesize more paxillin proteins. Moreover, they wanted to test the effects of both the hepatocyte growth factor (HGF) and the spingosine-1-phosphate (S1P) of a cell, which are how a cell develops and moves, and a factor in how permeable a cell is, respectively. They wanted to use both the HGF and the S1P as a factor to determine if what they were doing was effecting the cell at all. They tested mutated Y31 and Y118 amino acids in lung cells that reduced the production of paxillin. After
Other researchers had already determined that paxillin contributes greatly to the structural integrity of the outer membrane of a cell. For instance, paxillin leads to the formation of actin structures that are located at the edge of a cell, such as lamellipodia. What scientist don’t know is exactly how paxillin effects different functions in a cell. In order to solve this mystery, Cress, et al. wanted to take the research further and determine the role that paxillin played in lamellipodia formation, and how paxillin affected the barrier that surrounds a cell. This would be a step toward finding out how much of an effect that proteins have in the restoration of the outer barrier of a cell. If scientist could figure this out, another step could be taken to figure out how to use proteins to fix cells. They did not want to test this theory on all types of cells, so they chose to only focus on human lung microvascular endothelial cells, or HLMVEC for short. That means that this study could really only directly help with the study of damaged lung cells. They also wanted to focus on how paxillin was effected by Y31 and Y118, which are specific amino acids that are used by cells to synthesize proteins. The scientists needed to know, if their theory was correct and paxillin had an effect on the barrier of a cell, how to synthesize more paxillin proteins. Moreover, they wanted to test the effects of both the hepatocyte growth factor (HGF) and the spingosine-1-phosphate (S1P) of a cell, which are how a cell develops and moves, and a factor in how permeable a cell is, respectively. They wanted to use both the HGF and the S1P as a factor to determine if what they were doing was effecting the cell at all. They tested mutated Y31 and Y118 amino acids in lung cells that reduced the production of paxillin. After