The reactants that enzyme act upon with the substrate and the enzyme will bind together into the active site. The catalyst is important to the enzyme that breaks down the hydrogen peroxide and oxygen gas, where the rate of reaction of the catalyst will increase the temperature until it has reached the optimum temperature above 37°C is reached. If the optimum temperature has reached above 37°C then the kinetic energy and substrate molecules will begin to denature. The purpose of the lab was to determine the effects different temperature in yeast and hydrogen peroxide in a catalyst enzyme on reaction rate. There were many sources of errors that occur in the lab from having just one trial, using the same thermometer for each solution and inaccurate timing.…
Aim: This is an investigation to establish the effect of different temperatures (from 0°C -100°C) on the production of oxygen gas in a potato catalase reaction, using hydrogen peroxide as a substrate. The oxygen gas produced will be measured using a gas pressure sensor. Introduction: Enzymes are proteins, known as biological catalysts that increase the rate of reaction in a biochemical reaction without being chemically altered.…
The hypothesis was proven wrong by the timing and values of the graph. I believe this was caused by the enzyme having to break apart a heightened amount of substrates in the mixed test tubes. This caused the initial data to yield a quicker reaction (on the basest of browning in color) compared to the altered data. The independent variable of this experiment was time, while the dependent variable was the color as the reaction is completely dependent on the duration of time. There were numerous potentially sources of error during this experiment.…
INTRODUCTION: The objective of this lab is to measure the activity of an enzyme and the effects of environment conditions on enzyme activity. Enzymes are catalysts; agents that speed up chemical reactions by lowering the activation energy required. This means that a catalyst helps reactions occur at a greater speed and lower temperature.…
A chemical reaction’s rate is described by the amount of product formed. Substances that increase that rate are called catalysts. Catalysts are often proteins called enzymes. Enzymes change the pathway of the reaction between the products and the product. However, enzymes don’t alter the starting or ending points.…
The enzyme being used in this experiment is catalase. Catalase is an antioxidant enzyme that helps to…
The difference between the two graphs is the speed of the reaction. The temperatures of the two tests were very similar throughout the experiment. when doing this experiment one should have a paper towel ready to wipe the side of the test tube to be able to read the temperature. Conclusion: This experiment tested the difference between a untreated and treated catalase enzyme.…
The specificity of enzymes helps make them powerful tools in nature; they are allowed to form enzyme-substrate complexes. (Bioinfo.org.cn, 2015) Reaction rates controlled by enzyme can be measured using experimental methods where the factors such as enzyme, pH and temperature can be studied. These results can be…
After we did all three of the concentrations, we can conclude that all three of them increased their gas levels. However, 0% NaCl concentration increased the most because since it has no salt, it could work in its normal environment and the gas levels increased a lot and it was nowhere near getting denatured. In the 2% NaCl concentration, the gas levels increased, but not as much as 0% NaCl concentration because salt is known for denaturing enzymes over time, so as time went on the experiment, the enzyme starts getting denatured and you notice it in the end because the gas levels are increasing less and less. In the 10% NaCl concentration, the gas level increased, but not as much as 2% NaCl concentration because there is so much more NaCl, so the enzyme will get denatured even quicker and the gas level will increase less and less early on in the…
Through this experiment we measured how fast a chemical reaction occurs, by changing the degradation rate in Albumin when added to different enzymes. Albumin is a protein found in egg white, which is considered to have important storage and nutritional functions. Albumin has also been used in medicine to treat heavy metal intoxication. We ran Albumin through four different conditions to see which would make the Albumin degrade faster. We predicted that the Albumin would degrade with pepsin the fastest since it is essential for digestion of substances in the stomach.…
The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it took for the reaction to occur. To investigate the effect of the concentration of the substrate on the reaction time, four test tubes were used.…
However, when the substrate concentration was increased, the intensity of color stopped increasing due to the enzymes being unable to to process at a faster rate. All of the alternate hypothesizes were supported; in the experiments, section one (with Figure 1) had an optimal temperature at approximately 24°C; section two (with Figure 2) had an optimal pH at approximately pH8; in section 3 (with Figure 3) the color continued to increase with the addition of more enzymes; and in section 4 (with Figure 4) the color continued to increase until it reached a maximum velocity with the addition of more substrates. One desired retrial for the experiment would be to test for more points after 20 drops of potato juice in Figure 3. Understanding the effects of how these properties alter the ability of the catecholase enzyme could allow for better insight into decreasing time needed for biochemical reactions, proper food storage, and the condition of…
Lactate dehydrogenase (LDH) is an oxidoreductase found in both eukaryotes and prokaryotes. LDH catalyses the formation of lactate and NAD+ from pyruvate and NADH, during the last step of anaerobic glycolysis. The reaction is also catalysed in the other direction by LDH during the Cori cycle. This reaction can be assayed using spectrophotometric techniques; peak absorbance of NADH is at 340nm, but the peak absorbance of NAD+ is 259nm (Powers, et al., 2007). Enzyme assays are performed to measure the rate of an enzyme-catalysed reaction, known as the enzyme activity.…
Introduction: Enzymes have a huge effect on the human body and other organisms. Enzymes are catalysts that are used to accelerate the reaction process by lowering the activation energy. For this experiment the catechol oxidase was extracted from potatoes. The enzyme is called catechol oxidase because the oxygen is reacting to catechol. Catechol oxidase causes the browning of the fruits and vegetables because the compound is toxic to the bacteria.…
Purpose: To test the effect of inorganic and organic catalyst concentration and surface area on the reaction rate. Introduction: In this experiment the experimenters will seek to determine what factors determine the rate of a reaction with a catalyst. A catalyst is a molecule that starts a chemical reaction, but it is not part of the reaction it is causing.…