ABSTRACT
Objective: To evaluate the in-vitro anti-inflammatory activity on leaves extract of Callistemon citrinus by using inhibition of albumin denaturation technique. Method- The different extract i.e. chloroformic, ethanolic and aqueous extract at different concentrations was incubated with egg albumin in controlled experimental condition and subjected to determine absorbance to evaluate the anti-inflammatory activity. Diclofenac sodium was used as the standard/ reference drug. Result: This study revealed that the concentration dependent inhibition of protein (egg albumin) denaturation by the different extract. The effect of standard drug (Diclofenac sodium) was found to be less than that of test extract. Conclusion: From the present study it …show more content…
All the other chemicals were of analytical grade obtained commercially. Double distilled water from all-glass still was used throughout the study.
1.3. Preparation of extracts
The successive extraction of plant material was done by soxhlet apparatus by using different solvent such as petroleum ether, chloroform, ethanol and water. The extract was filtered and evaporated to dryness to yield the dry extract. The dry extract was kept in a vacuum desiccator until use.
1.4. Preparation of Test Samples
The varying concentrations of test samples (chloroformic, ethanolic and aqueous of callistemon citrinus) were prepared mainly 50, 150, 250, 500 and 1000μg/ml by using 10000 μg/ml as stock. {Prepared by dissolving 100 mg of material (test samples) upto 10ml with distilled water}.
1.5. Preparation of Standard
Similarly the varying concentrations of standard (Diclofenac sodium) were prepared mainly 50, 150, 250, 500 and 1000μg/ml by using 10000 μg/ml as stock.
1.6. Preparation of Phosphate buffered saline
Dissolve 28.80 gm of disodium hydrogen phosphate and 11.45 gm of potassium di hydrogen phosphate in sufficient water to produce 1000
Objective: To evaluate the in-vitro anti-inflammatory activity on leaves extract of Callistemon citrinus by using inhibition of albumin denaturation technique. Method- The different extract i.e. chloroformic, ethanolic and aqueous extract at different concentrations was incubated with egg albumin in controlled experimental condition and subjected to determine absorbance to evaluate the anti-inflammatory activity. Diclofenac sodium was used as the standard/ reference drug. Result: This study revealed that the concentration dependent inhibition of protein (egg albumin) denaturation by the different extract. The effect of standard drug (Diclofenac sodium) was found to be less than that of test extract. Conclusion: From the present study it …show more content…
All the other chemicals were of analytical grade obtained commercially. Double distilled water from all-glass still was used throughout the study.
1.3. Preparation of extracts
The successive extraction of plant material was done by soxhlet apparatus by using different solvent such as petroleum ether, chloroform, ethanol and water. The extract was filtered and evaporated to dryness to yield the dry extract. The dry extract was kept in a vacuum desiccator until use.
1.4. Preparation of Test Samples
The varying concentrations of test samples (chloroformic, ethanolic and aqueous of callistemon citrinus) were prepared mainly 50, 150, 250, 500 and 1000μg/ml by using 10000 μg/ml as stock. {Prepared by dissolving 100 mg of material (test samples) upto 10ml with distilled water}.
1.5. Preparation of Standard
Similarly the varying concentrations of standard (Diclofenac sodium) were prepared mainly 50, 150, 250, 500 and 1000μg/ml by using 10000 μg/ml as stock.
1.6. Preparation of Phosphate buffered saline
Dissolve 28.80 gm of disodium hydrogen phosphate and 11.45 gm of potassium di hydrogen phosphate in sufficient water to produce 1000