Biological Macromolecules Lab Report

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Biological macromolecules are large molecules made up of smaller organic molecules.

There are four classes of bio macromolecules : Carbohydrates, proteins, lipids, and nucleic acid. For carbohydrates and proteins these smaller molecules are known as monomers. These similar or identical monomers are covalently bonded together to create a large polymer molecule. Monomers, or base units are bonded together to create larger molecules via dehydration. This involves the removal of a water molecule at the bonding site. The larger molecule can be broken down by the reverse process, hydrolysis. This occurs when water is added to break the covalent bonds created during dehydration.

The monomer unit for carbohydrates is a monosaccharide or a simple
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Hot Plate 1/Bench with 500mL Beaker (300mL of Tap

Procedures:

PROCEDURE6.1:
Performing Benedict’s Test for reducing sugars:
1. Get Seventeen test tubes and label them from A1-­‐A17.
2. Add The materials to be tested in all of the test tubes and add about 2mL Of Benedicts Solution to each tube.
3. After Pouring Benedict’s Solution into each tube place all the test tubes in a boiling water bath for 3 min. Observe The color change.
4. After Observing the color change for 3 Min remove the test tubes from the boiling water tub and allow it to cool to the room temperature. 5. Record The color observations in table

PROCEDURE 6.2:
Performing IodineTest for starch:
1. Get Seventeen test tubes and label them from B1-­‐B17.
2. Add The testing materials in all of the test tubes.
3. Add Seven to ten drops of iodine to each tube.
4. Record The observations in table

PROCEDURE 6.3:
Performing Biuret Test for protein:
1.Get Seventeen test tubes and label them from C1-­‐C17.
2. Add The materials to be tested in each of the tubes.
3.In Each tube add 2ml Of 2.5% sodium hydroxide solution.
4.Add And mix 5 Drops of Biuret Reagent in each

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