Interactive Question 7.2 Cite some experimental evidence that indicates that membrane proteins drift. A good form of experimental evidence is Fluorescence Recovery After Photobleaching, aka FRAP. In the FRAP process, membrane proteins are labelled with a green fluorescent protein, and part of the plasma membrane is “bleached” with a laser, causing them to lose their fluorescence. The part that was bleached will eventually become fluorescent again, as molecules drift in by diffusion.…
The effect of the BsrG1 restriction enzyme on Wild Type and Mutation 1 of E. coli DNA Introduction: What a lac operon is involved in the lactose metabolism of E. coli and it can only work when there is no glucose present to interfere with the lactose metabolism. (Khan Academy) E. coli chooses prefers glucose and other "better" sugars, so if any are present other than lactose the lac operon will not be expressed in the plasmid. (Khan Academy) A plasmid is an "independent, circular, self-replication DNA molecule" that contrasts with the more complex helical shaped DNA of Eukaryotes.…
Results In the lab, 478 trials were conducted using a total of 956 crickets. The 478 pairs resulted in 264 wins for the resident cricket and 214 wins for the intruding crickets. These results produced a chi squared value of 5.230. The p-value calculated from this lab was .022.…
Materials. Numerous substances in the experiment were used. The most frequently used was the unknown due to the need to test its physical and chemical qualities. When a solution of the unknown was made, 1.000 g of the unknown and 1.0 mL of water was used to make it. To test for the possible ions, 1.0 mL of silver nitrate and 1.0 mL of nitric acid were used for the ion test.…
1) How should a urine sample be collected to ensure accurate laboratory results? As a nurse, what can you do to encourage this practice? A urine specimen can me collected in many ways: clean-catch method, by using a catheter, suprapubic, cystoscopy, and nephrostomy. Edith is a patient that could easily use the clean-catch method.…
Instructions: Buddy Lab - Physical and Chemical Change You’ve learned a lot about matter lately and now is your chance to teach someone younger about what you know. Your job is to design a lab that will help a primary student experiment and understand what physical and chemical changes are. Follow the guiding questions below to help you create your lab. Question:…
Mixed Unknowns This report discusses a series of tests along with results done in order to determine two bacteria in a given broth. For this experiment we were provided with an unknown broth, along with the supplies and media to perform the needed tests that would determine what bacteria the broth contained. We were also provided with a chart of possible bacteria. All tests were preformed with aseptic and sterile techniques.…
The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if it’s positive or negative. Performing aseptic techniques is the most crucial step that requires the utilizing of transferring, inoculating, and storing bacterial cultures and media. Aseptic technique is defined as procedures that prevent contamination…
It is important to note that the goal of this experiment was to find the pKa value of bromothymol blue, a pH indicator commonly used in titrations. [UNC, 2017] The pKa is where the number of moles of acid to conjugated base are equivalent. To find this, both Beer’s Law and spectroscopy were utilized in an attempt to make graphs to show just where this value occurred. According to Sigma-Aldrich, the pKa of bromothymol blue is approximately 7.10.…
Gel electrophoresis is a method used for separation and analysis of molecules such as DNA, RNA, and proteins, based on their sizes and polarity. DNA (deoxyribonucleic acid) is a molecule that carries most of our genetic information, and possesses a negative charge. During gel electrophoresis, DNA fragments can migrate through the gel also known as agarose when placed in a powerful electrical field. The rate at which the DNA fragments will move through the gel depends on their relative size. Horizontal gel slabs are commonly used on conducting gel electrophoresis.…
The aim of this project is to develop an Electrocardiography (ECG) amplifier circuit from scratch. The main challenges include amplifying the desired week signal in the presence of noise from other muscles and electrical sources. As this semester was about transistors we had to use transistors at some stage in this project. The project was successfully designed and built in two ways: one with solely using operational amplifiers and other using transistors as differential amplifiers. Electrocardiography (ECG)/ Background…
The phlebotomist may have a disease that the patient does not. The two individual’s blood may mix and the patient may get a false-positive result because of the phlebotomist’s blood mixing with the specialist ’s. Imagine the fear that would be experienced by getting a false result because of a little accident such as that. This is the ultimate reason why people in this profession must keep a clean practice.…
Preparation for Cell Lysis Obtain the mammalian expression vector for homo sapien ESR2 from Addgene. Assemble the vector to code for the proteins snail, slug and twist. Transfect HEK293 cells with the modified plasmids in petri dishes. Allow the cells to grow for 1-2 days so they will express the proteins. Transfer the culture medium to a centrifuge tube to separate it from the cells.…
Introduction In this lab report I use two different techniques to identify Unknown A and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia.…
INTRODUCTON Urinalysis is a test that evaluates a sample of urine. The urinalysis is used as a screening/or diagnostic tool because it can help detect substances or cellular material in the urine associated with different metabolic and kidney disorders. It is ordered widely and routinely to detect any abnormalities that require follow up. Often, substances such us protein or glucose will begin to appear in the urine before people are aware that they may have a problem. It is used to detect urinary tract infections (UTIs) and other disorders of the urinary tract.…