Aseptic Report

When conducting the different tests and streaking, aseptic techniques were followed. For every T-streaked that was done, the inoculating loop was first flame for 5 seconds and was allowed to cool for 30 seconds. Afterwards the tube containing the mixed unknown bacteria was then passed over the flame. The inoculating loop was then inserted into the broth and then carefully streak on quadrant one of the plate. Afterwards, the loop was sterilized by the flame and was allow to cool for thirty seconds so the next streak can be applied. The same concept was then applied for the different metabolic test. It is important to practice aseptic techniques to prevent contamination of results (3). A broth of mixed unknown bacteria was given and labeled …show more content…
It was then incubated at 37˚C for 48 hours. Afterwards the plate was observed for any color change and results were recorded (3). The Urease test was performed by heavily inoculating the broth, with a pure isolated bacteria colony from the gram negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 8 days. Afterwards the urea broth was observed for any color change and the result was recorded (3). The Methyl Red test was performed by inoculating the MR-VP broth, with a pure isolated bacteria colony from the gram negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 24 hours. After 24 hours 15 drops of the reagent, methyl red, was added to the MR-VP. After several minutes the results were observed and recorded (3). The Voges-Proskauer test was performed by inoculating the MR-VP broth, with a pure isolated bacteria colony from the gram-negative TSA plate, with an inoculating loop through a swirly motion. It was then incubated at 37˚C for 3 days. After 3 days, 15 drops of the reagent barrits A, and 5 drops of the reagent barrits B was added to the MR-VP broth. After one hour, results were observed and recorded