The treatments included a fresh sample from one individual, a fresh sample sample of pooled individuals, a sample from one individual that was stored for 24 hours, and a pooled sample from multiple individuals that was stared for 24 hours. Based on the results of this trial, whether or not the hens were successfully fertilized or not will give an indication as to whether or not a sample from one individual is as, if not, more effective than a pooled sample from multiple individuals, similarly if a sample that was stored for 24 hours is as effective. if not, more than a fresh sample at successfully fertilizing the hen.
The use of artificial insemination has a long history. The first, unofficially, documented idea of artificial insemination was brought up some time between 1425-1474 involving a human woman rather than on livestock. It was theorized by the citizens at the time that Henry IV, King of Castile, who was known as being impotent had completed an artificial insemination on his wife. When his Queen had a child after six years of marriage and a previous annulment as a result of his impedance the idea of artificial insemination was brought up. Unfortunately, later on the queen claimed that the child