Hannah Anderson
Biology 111-
Assignment #2-Properties of Water
1.) Hypothesis and Null Hypothesis:
Hypothesis: Adding starch will keep from diffusing across the membrane. On the contrary, amylase will cause the starch molecules to break apart into smaller molecules of glucose allowing diffusion of glucose and Lugol’s reagent across the dialysis tubing.
Null Hypothesis: Adding amylase to the beaker will have no effect on starch molecules and diffusion of the glucose and Lugol’s reagent across the dialysis tubing will not be present.
2.) Protocol
Two large beakers, one small beaker, two cut pieces of soaked dialysis tubing, dialysis tubing clamps, rubber bands, pipettes, a bottle of a-amylyse, a bottle of cooked starch, a bottle of Lugols …show more content…
The amylase was broken down by larger starch molecules into smaller glucose molecules, resulting in diffusion across the dialysis tubing’s selectively permeable membrane. The Lugol’s then diffused across the membrane as well, but the starch was unable to diffuse due to its large size. Looking at the control experiment, the contents of the dialysis tubing turned dark purple, almost black, and the beaker stayed pretty much the same color. The reason for the color change inside the tubing is that Lugol’s was able to diffuse across the tubing’s selectively permeable membrane, and the starch turned purplish black in the presence of Lugol’s. When no starch was able to get in contact with the Lugol’s in the beaker, the beaker stayed the same. This is due to starch’s inability to diffuse across the membrane of the tubing. Looking at the experimental procedure, after an hour, the content in the dialysis bag changed to a slight brownish orange color, and the beaker produced a slightly lighter appearance, but stayed mostly the same reddish orange color. The contents of the dialysis bag didn’t turn blackish purple this time because amylase broke down the starch and turned it into glucose. If there is no starch in the presence of Lugol’s there will be no dark colored content. The glucose test tells us that glucose was able to diffuse across the selectively permeable membrane of the dialysis tubing. We know this because we detected glucose in the beaker after an hour. If we had waited longer, the glucose test will show equal concentrations in both the beaker and the tubing. Overall, amylase aids in digestive activity by breaking down the large molecules of starch into smaller glucose molecule. This process allows the glucose to be diffused into our bodies for thoroughly, providing us with replenished
Biology 111-
Assignment #2-Properties of Water
1.) Hypothesis and Null Hypothesis:
Hypothesis: Adding starch will keep from diffusing across the membrane. On the contrary, amylase will cause the starch molecules to break apart into smaller molecules of glucose allowing diffusion of glucose and Lugol’s reagent across the dialysis tubing.
Null Hypothesis: Adding amylase to the beaker will have no effect on starch molecules and diffusion of the glucose and Lugol’s reagent across the dialysis tubing will not be present.
2.) Protocol
Two large beakers, one small beaker, two cut pieces of soaked dialysis tubing, dialysis tubing clamps, rubber bands, pipettes, a bottle of a-amylyse, a bottle of cooked starch, a bottle of Lugols …show more content…
The amylase was broken down by larger starch molecules into smaller glucose molecules, resulting in diffusion across the dialysis tubing’s selectively permeable membrane. The Lugol’s then diffused across the membrane as well, but the starch was unable to diffuse due to its large size. Looking at the control experiment, the contents of the dialysis tubing turned dark purple, almost black, and the beaker stayed pretty much the same color. The reason for the color change inside the tubing is that Lugol’s was able to diffuse across the tubing’s selectively permeable membrane, and the starch turned purplish black in the presence of Lugol’s. When no starch was able to get in contact with the Lugol’s in the beaker, the beaker stayed the same. This is due to starch’s inability to diffuse across the membrane of the tubing. Looking at the experimental procedure, after an hour, the content in the dialysis bag changed to a slight brownish orange color, and the beaker produced a slightly lighter appearance, but stayed mostly the same reddish orange color. The contents of the dialysis bag didn’t turn blackish purple this time because amylase broke down the starch and turned it into glucose. If there is no starch in the presence of Lugol’s there will be no dark colored content. The glucose test tells us that glucose was able to diffuse across the selectively permeable membrane of the dialysis tubing. We know this because we detected glucose in the beaker after an hour. If we had waited longer, the glucose test will show equal concentrations in both the beaker and the tubing. Overall, amylase aids in digestive activity by breaking down the large molecules of starch into smaller glucose molecule. This process allows the glucose to be diffused into our bodies for thoroughly, providing us with replenished