Material and Methods
Animal Care
Female Mus musculus, Swiss mice, were used in this experiment and purchased from Hilltop Lab Animals, Inc. in Reading PA. The mice varied in size, weighing approximately 12-40g. The mice were fed Purina Rodent Chow for Mice and given water ad libitum. The mice were acclimatized to 22°C, with an even photoperiod of 12 hours of light to 12 hours of dark, respectively. The mice were fasted 12 hours before the experiment.
Static Respirometry One at a time, the mice were placed into pre-massed chambers, 250 mL in volume. These were then massed to obtain the mass of the mice. Each chamber was placed into a water bath of particular temperatures. After being acclimated for 10 minutes, the O2 consumption of each mouse was measured and recorded using a LabPro data …show more content…
In Microsoft Excel, a scatterplot graph, with a trendline, was created and showed the linear equation with the R2 value, which represented the relationship between log mass and log MRMS. In Microsoft Excel, the mean O2 consumption per mass and standard deviation for how different-sized mice were affected by different temperatures, shown as MRMS values, were represented by a bar graph, with error bars representing the standard deviation for each temperature acclimation. Two slope t-tests were performed to determine a significance between the known slopes, of -0.25 and -0.33, and the experimental slopes. Using nonparametric repeated measures ANOVA, a Friedman Test was run to conclude a difference between the mean values of MRMS at the different temperature acclimations and whether the difference was significant for both, large and small groups. Then a Dunn’s Multiple Comparisons test was run to conclude a significant difference among any means within the same weight group. For each test, there was a significant difference if P<0.05 was found. All three tests were performed in Graphpad’s Instat
Animal Care
Female Mus musculus, Swiss mice, were used in this experiment and purchased from Hilltop Lab Animals, Inc. in Reading PA. The mice varied in size, weighing approximately 12-40g. The mice were fed Purina Rodent Chow for Mice and given water ad libitum. The mice were acclimatized to 22°C, with an even photoperiod of 12 hours of light to 12 hours of dark, respectively. The mice were fasted 12 hours before the experiment.
Static Respirometry One at a time, the mice were placed into pre-massed chambers, 250 mL in volume. These were then massed to obtain the mass of the mice. Each chamber was placed into a water bath of particular temperatures. After being acclimated for 10 minutes, the O2 consumption of each mouse was measured and recorded using a LabPro data …show more content…
In Microsoft Excel, a scatterplot graph, with a trendline, was created and showed the linear equation with the R2 value, which represented the relationship between log mass and log MRMS. In Microsoft Excel, the mean O2 consumption per mass and standard deviation for how different-sized mice were affected by different temperatures, shown as MRMS values, were represented by a bar graph, with error bars representing the standard deviation for each temperature acclimation. Two slope t-tests were performed to determine a significance between the known slopes, of -0.25 and -0.33, and the experimental slopes. Using nonparametric repeated measures ANOVA, a Friedman Test was run to conclude a difference between the mean values of MRMS at the different temperature acclimations and whether the difference was significant for both, large and small groups. Then a Dunn’s Multiple Comparisons test was run to conclude a significant difference among any means within the same weight group. For each test, there was a significant difference if P<0.05 was found. All three tests were performed in Graphpad’s Instat