Analysis Of Enzyme Lab Report

Great Essays
Emily Melendez
January 31, 2017
BSC2010
Enzyme I: pH and Temperature Lab Report
Introduction:
(2008, November 11). Without Enzyme, Biological Reaction Essential To Life Takes 2.3 Billion Years. Retrieved January 31, 2017. The basic concept that is being explored are about enzymes. Enzymes are proteins that are known for being sparkplugs in a chemical reaction. Enzymes have a shape and for them to ‘spark’ they must have the proper substrate for it to work U. This does not change the enzyme’s makeup but instead gives a resulting product. The shape of the enzyme is connected in relation to its function. The function of the enzyme can play a significant role in our environment. The conditions of our environment can change the shape of the enzyme
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We posed a question and we predicted that the presence of an enzyme may be affected due to the addition of water. From personal experience in chemistry, adding water to a compound like pNpp results in it breaking down the phosphate ion from its original compound. Breaking down the compound results in a quick change in reaction which results in faster rate of change. Therefore, we hypothesized that the presence of an enzyme may speed up due to the addition of water.
For the second experiment, we measured the effect of pH on the rate of reaction of BAP. We posed a question and predicted that the pH will affect the rate of BAP activity. The pH effects and changes the structure of the enzyme. The shape of the enzyme is what makes up function of the enzyme. From the lab information, it discusses about how the pH effects the enzyme. The video for the pre-lab states that adding H+ ions or OH- ions tends to increase the rate of an enzyme. Therefore, we hypothesized that the pH increases the rate of BAP by adding H+ ions or OH-
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In the information given, the enzymes are said an influence of environmental conditions could affect the enzymes rate of reactions. The results showed that the enzyme did indeed get affected by the temperature and the ph. My conclusion also reinstated that two of my hypotheses were correct under the information I was given in class about. The information really touched a lot of basis on the background of enzyme BAP and the function of which the enzyme does for its substrate to make a proper product.
The next question I would want to ask now if it the enzyme would in different variations of water from different environments. For an example, pond water and ocean water. Would the enzyme be able to react quicker or slower with different variations of water? In the experiment, we used distilled water and changed the pH and temperature. What if you used a different kind of water? Would that effect it in some

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