pH meter: Weighing balance. Materials:
HPLC grade Methanol, HPLC grade analytical water, Acetonitrile, Membrane filter paper- 0.45µm was used.
Selection of Detection wavelength: Individual dilutions of Dapagliflozin and Saxagliptin were prepared from standard stock solutions using methanol. Both the solutions were scanned over the range of 200-400nm on UV-VIS spectrophotometer, using medium scan speed. From the overlay spectra, using isobestic point (which is the wavelength where the molar absorptivity is the same for two substance that are inter- convertible), 210 nm has been selected as the detection wavelength for HPLC method. This wavelength was selected and at this wavelength, the response of the drugs was adequate.
Chromatographic condition:
The mobile phase consists of mixture of methanol and water in 80:20 v/v ratio. The mobile phase filtered through the membrane filter and degasssed. The mobile phase pumped through the column at 1ml/ min flow rate. The column temperature maintained at ambient Condition. The eluents was determined at 210 nm. Optimized condition s was shown below the