A Comparative Analysis Of Cilnidipine And Chlorthalidone

A simple, economic, selective, precise, accurate and sensitive HPTLC method for analysis of Cilnidipine and Chlorthalidone, as a bulk drug and in formulation, was developed and validated according to ICH guidelines. The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase while the solvent consisted of Toluene: Methanol: Ethyl acetate (8:2:0.1 v/v/v). The system was found to give compact spot for drug (Rf value of Cilnidipine and Chlorthalidone was 0.75 and 0.47 respectively). Densitometric analysis of Cilnidipine and Chlorthalidone were carried out in the absorbance mode at 230 nm. The linear regression analysis data for the calibration plots of Cilnidipine and Chlorthalidone showed good linear relationship with r2 = 0.991 for CILN and r2 =0.997 for CHLO , with respect to peak area in the concentration range 800-2400 and 1000-3000 ng per spot, respectively. The method was validated for precision and recovery. The limits of detection and quantitation for Cilnidipine were 17.18 and 52.06 respectively and for Chlorthalidone, were 48.33 and 146.4 respectively. The precision (intra-day, inter-day) of method were found within limits (RSD <2%). Thus the proposed method was simple, precise, economic, rapid and accurate and can be successfully applied for simultaneous estimation …show more content…
2] is a thiazide diuretic. It works by increasing the efficacy of the kidneys in removing excess salt and water from the bloodstream, which lowers blood pressure[11]. Chemically it is RS)-2-chloro-5-(1-hydroxy-3-oxo-2,3-dihydro-1H-isoindol-1-yl)benzene-1-sulfonamide[12].. This drug is official in Indian Pharmacopoeia[3], United State Pharmacopoeia[4], British Pharmacopoeia[5] and European Pharmacopoeia[6]. This all Pharmacopoeia describes UV, RP-HPLC and TLC methods for its estimation. Literature survey reveals that UV[13] and HPLC[14, 15] methods have been reported for its determination in single and combined dosage