Antibiotic sensitivity is the susceptibility of bacteria to antibiotics. Susceptibility can vary within bacteria of the same species due to different strains; with some strains being more resistant than others. Antimicrobial resistance is the ability of a microorganism to survive and multiply in the presence of an antimicrobial agent that would normally inhibit or kill this species of microorganism. Antibiotic susceptibility tests are done in order to determine, which specific antimicrobials will inhibit the growth of bacteria or fungi that causes the infection.
The aim of these tests is to predict the in vivo success or failure of the antibiotic therapy. The tests are carried out in vitro, and performed under standardized conditions so that …show more content…
There are a number of sensitivity tests available, with their respective advantages and disadvantages. Each test has their own strengths and weaknesses, including organisms that maybe accurately tested by the method. Some of the tests produce quantitative results, such as, minimum inhibitory concentration (MIC), whereas other sensitivity tests provide qualitative assessments, using categories susceptible, intermediate, or resistant. Antibiotic sensitivity test results can be a useful guideline in selecting the best antibiotic treatment option for each particular …show more content…
Types of Antibiotic Sensitivity Tests
1. Dilution Method
Broth Dilution: this method involves exposing isolates, to a series of concentrations of antimicrobial agents in a broth environment. For this method, the lowest concentration at which the isolate is completely inhibited is recorded as the minimal inhibitory concentration (MIC). The test is only valid if the positive control shows growth, and the negative control shows no growth.
Agar Dilution: this method has the same procedure as the broth dilution. Agar dilution follows the principle of establishing the lowest concentration of the consecutively diluted antibiotic concentration at which bacterial growth is still inhibited.
Advantage: group of minimal inhibitory concentrations, the result reproducibility, convenience of having pre- prepared panels, economy of reagents, test requires less space, and also provides assistance in generation computerized reports if an automated panel is
The aim of these tests is to predict the in vivo success or failure of the antibiotic therapy. The tests are carried out in vitro, and performed under standardized conditions so that …show more content…
There are a number of sensitivity tests available, with their respective advantages and disadvantages. Each test has their own strengths and weaknesses, including organisms that maybe accurately tested by the method. Some of the tests produce quantitative results, such as, minimum inhibitory concentration (MIC), whereas other sensitivity tests provide qualitative assessments, using categories susceptible, intermediate, or resistant. Antibiotic sensitivity test results can be a useful guideline in selecting the best antibiotic treatment option for each particular …show more content…
Types of Antibiotic Sensitivity Tests
1. Dilution Method
Broth Dilution: this method involves exposing isolates, to a series of concentrations of antimicrobial agents in a broth environment. For this method, the lowest concentration at which the isolate is completely inhibited is recorded as the minimal inhibitory concentration (MIC). The test is only valid if the positive control shows growth, and the negative control shows no growth.
Agar Dilution: this method has the same procedure as the broth dilution. Agar dilution follows the principle of establishing the lowest concentration of the consecutively diluted antibiotic concentration at which bacterial growth is still inhibited.
Advantage: group of minimal inhibitory concentrations, the result reproducibility, convenience of having pre- prepared panels, economy of reagents, test requires less space, and also provides assistance in generation computerized reports if an automated panel is