Adjusting Hydrogen Peroxıde Colloıdal-Ag Case Study

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The purpose of this study was to investigate the effect of a system that automatically adjusting hydrogen peroxıde Colloıdal-Ag dose to reduce colonization and growth of heterotrophic bacteria in dental unit waterlines and wastewaters. Thirty dental units were assigned into three groups with respect to the protocol employed: Group 1: 10 units were selected for automatic administration. Group 2: 10 units were selected for manual administration and Group 3: 10 units served as controls. Water samples from DUWLs and waste waters were collected every week during the ten weeks. They were inoculated onto various specific agar media and incubated at 28-37ºC for 2-7 days. After counting the colonies of microorganisms, they were identified and the mean …show more content…
In a series of trials, treating of DUWLs with sodium hypochlorite, glutaraldehyde, and isopropanol was investigated. These agents reduced microorganisms in effluent water but did not destroy the biofilm matrix in the DUWLs, even with periodic treatments [ 8].Similar results were obtained for Listerine, Bio 2000, Rembrandt, and Sodium fluoride [ 23].There are now an increasing number of commercial disinfectants that practice managers can use to reduce the microbial burden of their DUWLs and thereby minimize the risk of occupational exposure and cross-infection. A number of studies have evaluated a broad range of products, suitable for use in DUWLs, which are claimed to reduce the microbial loading of dental unit water systems and to remove the biofilm attached to the inner surfaces of the dental unit water line tubing [13-15, 24].The studies that investigated the effect of hydrogen peroxide silver ions disinfectant have shown that it is easy to use and continuous application of this agent caused a significant decrease both in the number of total fungi and 91% of the treated water samples had a TVC of ≤ 200 cfu/mL [13,19,25]. In this study; hydrogen peroxide colloidal-Ag was used to reduce total viable counts in DUWLs and similar …show more content…
Identifying environmental reservoirs of methicillin-sensitive Staphylococcus aureus (MSSA), as well as Methicillin-resistant Staphylococcus aureus (MRSA) in the community might be critical for controlling the spread of community–acquired staphylococcal infections [27]. Dental unit waterlines are known as reservoirs since they are frequently contaminated by pathogenic and opportunistic microorganisms and [28] the contamination of dental unit waterlines by MSSA strains is also important as MRSA strains because they are accepted as being a causative agent for the community-acquired infections and recently, the incidence of these types of infection are getting very much higher. In the waste water samples we have found MSSA strains so the occurrence of MSSA must be paid attention to a probable way of contamination to people who are in contact with reclaimed wastewater. Previous studies investigated for the hospitals the effect of detergents, organic compounds, disinfectants and several metals [29,30]. But there isn’t any data on the topic of dental unit waste waters microbiological compounds and counts. One of the aim of this study was to investigate the effect of hydrogen peroxıde Colloıdal-Ag on dental unit waste waters. According

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