INTRODUCTION
Our nerve cells are highly involved in the environmental and internal complex processes of our body. In order for the input and output mechanisms to go smoothly, these highly specialized cells, called neurons, must communicate not only with one another, but also with other muscles, and organs in an effective way throughout the body using electrical synapses. While neurons are made up off a cell body referred to as the soma, dendrites, and axon. Nerves consist of axons and sometimes nerve fibers, and each of those axons has the ability to produce an action potential. Action potential is a well-preserved message as a response to a specific stimulus that travels down the axon. Moreover, sodium/potassium pumps are located in the …show more content…
the voltage was set at 10 mV .we tested the nerve by a constant increase of 10mV till there was constant data on the amplitude that stopped it from increasing
Refractory Periods
in the 2nd experiment to determine the refractory period 2 stimuli were send to the nerve by decreasing the interval between them both every time at 200mV .
Conduction Velocity the third experiment was used to measure the conduction velocity of the nerve a double voltage of the 2nd experiment was entered in the stimulator panel . there was 2 data recorded proximal channel from the nearest electrode and the distal channel from the further electrode. the distance was measured in mm between the negative leads of both electrodes. time interval was measured between the nearest and the further compound action potential.
Results:
Eliciting the CAP: stimulus and action potential threshold of the frog sciatic nerve at 10.mV a threshold was observed of the frog sciatic nerve. with each voltage increase there was an increase in the amplitude of the CAP. the maximum stimulus at 40 mV was reached at 1.90 mV. (there was no increase recorded after that for the CAP amplitude …show more content…
the 3.5ms is the actual end of the relative refractory period in my view since there was a significant decrease in the amplitude.the sodium channels are completely inactivated in the refractory period and it happens after a firing potential.in this experiment we conclude that a stimulus can generate very quickly after a previous action potential stimulus has ended.
Conduction velocity the conduction velocity was measured to be 29.77m/s a bit lower than the normal range between 50 to 60 m/s. this error could be due to the ringer’s solution if it contacted the wires resulting of short stimulus electrodes. another possibility is a conversion error of the program itself . If the measure in milliseconds was actually in seconds, the conduction velocity would have resulted in a lower measured velocity. Then the average fiber diameter would have been about 4µm according to the equation V=2.5D, where D = diameter of the fiber in micrometers (Animal Physiology Lab Manual, 2015)
Our nerve cells are highly involved in the environmental and internal complex processes of our body. In order for the input and output mechanisms to go smoothly, these highly specialized cells, called neurons, must communicate not only with one another, but also with other muscles, and organs in an effective way throughout the body using electrical synapses. While neurons are made up off a cell body referred to as the soma, dendrites, and axon. Nerves consist of axons and sometimes nerve fibers, and each of those axons has the ability to produce an action potential. Action potential is a well-preserved message as a response to a specific stimulus that travels down the axon. Moreover, sodium/potassium pumps are located in the …show more content…
the voltage was set at 10 mV .we tested the nerve by a constant increase of 10mV till there was constant data on the amplitude that stopped it from increasing
Refractory Periods
in the 2nd experiment to determine the refractory period 2 stimuli were send to the nerve by decreasing the interval between them both every time at 200mV .
Conduction Velocity the third experiment was used to measure the conduction velocity of the nerve a double voltage of the 2nd experiment was entered in the stimulator panel . there was 2 data recorded proximal channel from the nearest electrode and the distal channel from the further electrode. the distance was measured in mm between the negative leads of both electrodes. time interval was measured between the nearest and the further compound action potential.
Results:
Eliciting the CAP: stimulus and action potential threshold of the frog sciatic nerve at 10.mV a threshold was observed of the frog sciatic nerve. with each voltage increase there was an increase in the amplitude of the CAP. the maximum stimulus at 40 mV was reached at 1.90 mV. (there was no increase recorded after that for the CAP amplitude …show more content…
the 3.5ms is the actual end of the relative refractory period in my view since there was a significant decrease in the amplitude.the sodium channels are completely inactivated in the refractory period and it happens after a firing potential.in this experiment we conclude that a stimulus can generate very quickly after a previous action potential stimulus has ended.
Conduction velocity the conduction velocity was measured to be 29.77m/s a bit lower than the normal range between 50 to 60 m/s. this error could be due to the ringer’s solution if it contacted the wires resulting of short stimulus electrodes. another possibility is a conversion error of the program itself . If the measure in milliseconds was actually in seconds, the conduction velocity would have resulted in a lower measured velocity. Then the average fiber diameter would have been about 4µm according to the equation V=2.5D, where D = diameter of the fiber in micrometers (Animal Physiology Lab Manual, 2015)