1-Propanol Experiment

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In this experiment we used the alcohols methanol, ethanol, and 1-propanol, and tested the beet membranes at 0% alcohol concentration, 10% alcohol concentration, 20% alcohol concentration, 30% alcohol concentration, and 40% alcohol concentration. We used 0.5cm3 beet pieces throughout the entire experiment to keep the surface area constant in each alcohol. We then submerged one cube of the beet in each concentration of each alcohol for 15 minutes. We then found the concentration of Betacyanin in each alcohol solution by measuring the absorption in the spectrophotometer. The higher the absorbance, the higher presence of Betacyanin pigments in the alcohol solution. My results for the 1-propanol solutions have an ascending absorbance from 0.001 to 0.364; however, this is after omitting the 40% alcohol concentration that has an unusual absorbance of 0.220. …show more content…
The methanol solutions have ascending absorbance from 0.055 to 0.230; however, this is after omitting the 0% alcohol concentration that has an unusual absorbance of 0.065. Overall, according to my graph, the alcohol that did the most damage to the cell membrane was the 1-propanol. The 1-propanol ended up having the steepest line of best fit on its graph, which means that on average the 1-propanol caused the most destruction to the cell membrane. To come to this conclusion, I not only used quantitative data but I also used qualitative data. This is because the concentrations for the 1-propanol seemed to have the most intense red color in their concentrations. Then according for my graphs and lines of best fit that overall the ethanol cause more damage to the beet cell membranes than the methanol did. That’s means the methanol caused the least amount of destruction to the cell membranes. The amount of destruction each alcohol does can be explained by their molecular

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