López, C. Sánchez, R. Batlle, and C. Nerín from Spain in 2005, they conducted the antimicrobial activity of clove on several gram negative bacterium E. Coli, Yersinia enterocolitica,Salmonella choleraesuis, Pseudomonas aeruginosa, and several gram positive bacterium, S.aureus, Bacillus cereus, Entero faecalis, and Listeria monocytogenes. The clove essential oil was supplied by ARTIBAL. The compounds in clove essential oil was identified using the database of gas chromatography ion trap mass spectrometer (GC-IT-MS). The antimicrobial activity test was performed using solid diffusion assays vapor diffusion assay and MIC after incubation period. Solid diffusion assay was performed by inoculating 100μL of the solution with E.coli and S. aureus into the respective petri dish. The clove essential oil in the filter disk was placed onto the cultured media. P. López, C. Sánchez, R. Batlle, and C. Nerín reported that the clove essential oil which contained Eugenol has antimicrobial activity against S. aureus and E.coli. Based on Table 3 and Figure 4, the total inhibitory measured in solid diffusion array for S. aureus is higher than E.coli. The MIC value represents the lowest concentration of essential oil to inhibit the growth of microorganism. The MIC values for both bacteria is 26.6 μL/L which showed inhibition of
López, C. Sánchez, R. Batlle, and C. Nerín from Spain in 2005, they conducted the antimicrobial activity of clove on several gram negative bacterium E. Coli, Yersinia enterocolitica,Salmonella choleraesuis, Pseudomonas aeruginosa, and several gram positive bacterium, S.aureus, Bacillus cereus, Entero faecalis, and Listeria monocytogenes. The clove essential oil was supplied by ARTIBAL. The compounds in clove essential oil was identified using the database of gas chromatography ion trap mass spectrometer (GC-IT-MS). The antimicrobial activity test was performed using solid diffusion assays vapor diffusion assay and MIC after incubation period. Solid diffusion assay was performed by inoculating 100μL of the solution with E.coli and S. aureus into the respective petri dish. The clove essential oil in the filter disk was placed onto the cultured media. P. López, C. Sánchez, R. Batlle, and C. Nerín reported that the clove essential oil which contained Eugenol has antimicrobial activity against S. aureus and E.coli. Based on Table 3 and Figure 4, the total inhibitory measured in solid diffusion array for S. aureus is higher than E.coli. The MIC value represents the lowest concentration of essential oil to inhibit the growth of microorganism. The MIC values for both bacteria is 26.6 μL/L which showed inhibition of