The interest in the bacteria stems from its relevance to the cancer problem, that animal and plant cancer have many common characteristics. The disease it induces provides investigators with a model system in which fundamental aspects of bacterial infection, host-parasite relationships as well…
All of the isolates shown in Table 1 were resistance to S. aureus and were considered antibiotic producers. However, only isolate 12 and 16 showed resistant to the unknown Gram-negative organism. All of the antibiotic producers were then tested against Enterococcus faecalis, Pseudomonas putida, Acinetobacter baylyi, Escherichia coli, and Enterobacter aerogenes by the streak-plate method, which can be seen in Table 2. As demonstrated in Table 2, none of the antibiotic producers were found to be resistant against Enterococcus faecalis or Pseudomonas putida. Table 2 also showed isolates 3 and 4 were resistant to Acinetobacter baylyi, isolate 12 was resistant to Escherichia coli, and isolates 12 and 16 were resistant to Enterobacter aerogenes.…
acidovorans (2) matched the isolate bacteria; however, blast sequence only identified a highest of 88% 16S rRNA gene with max score base match of 636 bases. For a significant identification, 97% value would be needed. The MIC assay showed the isolate being resistant to all concentrations of ampicillin as the bacteria grew and formed biofilms in each one. For the DDA, the isolate bacteria did not grow abundantly throughout the ampicillin or the positive control as expected, although, it did have susceptibility to ciprofloxacin. However, this susceptibility became weaker as the first DDA was incubated for a longer period and a few colonies grew in the ciprofloxacin zone of inhibition.…
Although it was determined that the activity of antibiotics was inconclusive no raised colonies were visible although then nutrient agar appeared to be texture. Errors were made when plating. When the first molten agar was poured, the agar did not solid uniformly due to agitation. Also, when the Streptomyces was played a separate plate for the control Streptomyces and comparison of experimental Streptomyces and control…
Using a wire loop, a small sample of the unknown test tube was plated on a nutrient agar using the streak method . The unknown test tube was then placed in a refrigerator to slow down any continued growth. The nutrient agar was labeled “VB TR1” and was placed in an incubator for 48 hours at 37 degrees Celsius. Following this procedure, I started to do research on microorganisms that presented the same type of symptoms (diarrhea and nauseous). After 48 hours, the plate was observed.…
Elizabeth Casey Lab Partners: Cara Hull, Erin Magennis, Claire Pfeffer Bio2300 Section 1 10/6/16 Dr. Henle Testing Effects of Glucose and Sodium Chloride on E.coli Cell Division Hypothesis: We hypothesized that if E.coli cells are grown in broth containing 1% 1M glucose, then they will divide more rapidly than they would without the glucose additive. Our independent variable was the glucose, and our dependent variable was E.coli cell density. Methods: This procedure was obtained from the Growth Kinetics in Bacterial Cell Culture in the Cell Biology Lab Manual of Carthage College (2016).…
RESULTS Transformation: the transformation of the ade2 gene to the kanamycin resistant gene (ade2::kan2R) cause the cell to become red and grow on mediums containing G418 or kanamycin resistant mediums was observed to have occurred. PCR and Gel Electrophoresis: Figure 1 is the product of gel electrophoresis containing the wild type and transformed PCR products, either being or not being cut by HindIII. From the left (reader’s left) of the gel to the right the lanes are; 1.Ladder, 2. transformed PCR product, 3. transformed PCR product cut with HindIII, 4.…
Aileen San BIO 210L – Section 4 A Prokaryotic System for Transformation, Expression, and Purification of Eukaryotic Proteins Introduction: The bacteria E.coli is found in many places such as in animal intestines and the environment. These bacteria have a simple structure and are quick to reproduce (Centers for Disease Control and Prevention, 2015). Because of this, scientists know a lot about E. coli. In this experiment, the E. coli will be exposed to a pGLO plasmid; each plasmid has an Ori, pBAD, araC, bla, and GFP.…
The plate labeled LB/AMP/X-gal-plasmid and the plate labeled LB-plasmid display that AMP (ampicillin) and X-gal prevent cells without plasmid from growing. The necessary factors for bacterial growth are growing room, appropriate temperature, and minerals. Likewise, any substance that hinders growth will be malignant to the plasmid. In this experiment, that substance was ampicillin.…
This created a diffusion process that pumped growth factors and nutrients through the chambers into the isolated soil sample allowing the isolated uncultured bacteria to grow. After a colony was produced it was screened for antimicrobial activity on plates containing S. aureus. The screening research found that a beta-proteobacteria named Eleftheria terrae showed good activity. However, 16S rDNA and in silico DNA/DNA hybridization showed the researchers that the organism was related to the known genus Aquabacteria, a Gram-negative organism which is not an antibiotic producer. Conversely, when researchers used mass spectrometry on the sample, they found that part of it had an unusual molecular mass of 1,242 Da.…
There are three main mechanisms through which bacteria can develop resistance against beta-lactams, namely,developing PBPs with lower affinity for beta-lactam, reducing access to PBPs, or developing enzymes which act against the antibiotics, known as beta-lactamases (Rice 2012). The first method,that of producing new PBPs is most common in gram-positive bacteria, such as Staphylococcus aureus, which produces PBP2a,or Enterococcus faecium, which produces PBP5 (Rice 2012). Gram-negative bacteria however favour the production of beta-lactamases (Rice 2012). These enzymes9e similar in structure to PBPs, and so are able to catalyse the hydrolisation of the beta-lactam ring, rendering the antibiotic ineffective (Andersson, Terwisscha, & Valegard…
contain the genetic information that codes for the synthesis of Beta-Lactamase an enzyme which inactivates the β-lactam ring via hydrolysis causing a resistance to ampicillin (Li, Chan, & Chen, 2016). Therefore, Aeromonas spp. with this resistance no this organism can grow in the presence of…
The hypothesis about the +pGLO plate containing ampicillin and LB was correct because the bacteria did end up growing and not glowing under UV light. It ended up growing because the LB that was in the plate with it helped the bacteria grow and reproduce. The bacteria became resistant to the ampicillin because the DNA plasmid contained beta lactamase which made the cell resistant to ampicillin. It did not end up glowing under UV light because the gene for the green fluorescent protein was repressed by the araC gene found on the DNA plasmid. The hypothesis about the +pGLO plate containing LB, ampicillin, and arabinose was correct because the bacteria did end up growing and glowing under UV light.…
Abstract This experiment was constructed to distinguish the differences with Prokaryotes and Eukaryotes by growing through a variety of media. These media consisted of rich and minimal. Rich media is a media that contains an abundant supply of nutrients for bacteria to grow. Minimal media is a media that contains the minimal amount of nutrients for bacteria to grow.…