The food matrices are divided into food of animal origin and related products, vegetables, fruits and related compounds, and other food-related matrices.
HILIC is used on a large scale in the separation of different food matrices. In the recent years the use of HILIC has grown and the following figure relates to the same. Fig. 1.
Evolution of the published works in the last decade in food analysis using HILIC (data up to end of January 2011). The sources of information were the databases ISI-Web of Knowledge, Scirus, and Scopus. The search has been done using as keywords [(Hydrophilic Interaction Chromatography) or (HILIC)] and [(Food) or (Beverages) or (Drinks) or (Vegetables) or (Fruits)]. Fig. 2.
Summary …show more content…
FOOD OF ANIMAL ORIGIN
1. Dipeptides derived from β-alanine and histidine, like carnosine and its methylated analogues anserine and balenine that are widely distributed in the heart, tissues and the central nervous system of Vertebrates are analysed using HILIC separation method. This analysis used the matrix meat and same type of silica HILIC column was used. Detector used is DAD.
Mora et al. employed a simple and fast method without the need for complex clean up or sample derivatization. It involves analysis of carnosine, anserine, balenine, creatine, and creatinine in pork and chicken using a silica HILIC column.
(A) Chromatogram of the HILIC separation of carnosine (CAR), anserine (ANS), balenine (BAL), creatine (Cr), and creatinine (Cn).
(B) Chromatogram corresponding to a pork loin sample.
(C) Chromatogram corresponding to a chicken breast sample. In all cases CAR, ANS, balenine BAL, and Cr were detected at 214 nm (solid line), whereas Cn was detected at 236 nm (dotted line). Reprinted with permission from [25]. Copyright 2007 American Chemical …show more content…
FOOD AND BEVERAGES OF VEGETABLE ORIGIN
1. Furosine, has been analyzed not only in animal food but also in processed cereals and crisp bread with a silica HILIC column and DAD[26]γ-aminobutyric acid (GABA), the chief inhibitory neurotransmitter in the mammalian central nervous system and the compound directly responsible for the regulation of muscle tone, was quantified without derivatization in apple, orange juice, potato, and soybeans employing a zwitterionic HILIC stationary phase and MS/MS detection [29]
2. Pesticides have been frequently studied in vegetables, and ESI-MS/MS has been the most commonly employed analytical tool in their determination (see Table 2). Dithiocarbamate (DTC) fungicides are widely used in agriculture and horticulture due to their broad spectrum of activity, low acute mammalian toxicity, and low production costs. This group of fungicides has been analyzed with a ZIC-pHILIC stationary phase in combination with a quick, easy, cheap, effective, rugged, and safe extraction method (QuEChERS) for the extraction of propylenethiourea in tomato [30].
3. A HILIC silica column was successfully used to retain and separate streptomycin (an antibiotic) in mellow apples [31] and two quaternary ammonium growth regulators in fresh fruit, juices, beer, mushrooms, vegetables, bread, and coffee powder